Fig. 2. ^Ser51P‐eIF2α/eIF2α ratio and GADD153 protein expressions in Tacrolimus-, Sirolimus- and Everolimus-treated HepG2 (A and B, respectively) and Huh7 (C and D, respectively) cells. Treatments were administered at different concentrations (0, 10 nM, 10 µM, and 100 µM). The expression of ^Ser51P‐eIF2α and eIF2α (3 h), and GADD153 (12 h) was evaluated by Western‐blot analysis as described in Material and Methods. Results are expressed as mean ± SEM, and blots are representative of five independent experiments. *p ≤ 0.05 and **p ≤ 0.01 between control and immunosuppressant‐treated cells. The groups with different letters (a, b, c or d) were significantly different (p ≤ 0.05).